Figure 1. Triobp gene structure, transcripts, protein isoforms, and mutant alleles.

(A) Alternative transcripts of mouse Triobp, the corresponding encoded protein isoforms, and their predicted domains. Locations of the epitopes for 2 antibodies (TRIOBP-4/5 and TRIOBP-5) are depicted with orange and light-blue rectangles, respectively. (B) Drawing of an organ of Corti sensory epithelium segment showing 1 row of inner (IHC) and 3 rows of outer (OHC) hair cells. Inset shows Deiters’ and outer pillar cells supporting an OHC. (C) Mutations of Triobp used to generate 2 genetically different TRIOBP-5–deficient mouse models. Triobp^{ΔEx9-10/ΔEx9-10} has a LacZ cassette replacing exons 9 and 10 of Triobp-5 (schematic 1). The Triobp^ΔEx8 allele has a LacZ cassette replacing exon 8 (schematic 2) and the Triobp^YHB226 allele has a trap cassette with a LacZ insertion in exon 17 (schematic 3). The combination in trans of these 2 Triobp mutations is a compound heterozygote designated Triobp^ΔEx8/YHB226. Expression of wild-type Triobp isoforms 1, 4, and 5 is indicated to the right of the schematics for each genotype.