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. 2019 Jul 11;4(13):e128034. doi: 10.1172/jci.insight.128034

Figure 4. Mif expression correlates with higher parkin levels in lung lysate preinfection.

Figure 4

Lung lysate parkin/β-actin protein measured by Western blot at baseline and 3 DPI: (A) IFN-λ levels in 1 DPI lung lysate. Lung lysate protein measured by Western blot at 1 DPI: (B) parkin normalized to β-actin. (C) Representative image of blot. (D) Violin plot of parkin normalized to β-actin. (E) A representative image of blot. Statistical differences were determined by 1-way ANOVA followed by Tukey multiple comparisons test (D) or 1-way ANOVA comparing the mean of each group to Mif+/+ data followed by Holm-Sidak test for multiple comparisons (A and B). The violin plots are closed curves representing data distribution and encapsulate the median, range, and interquartilerange, with each symbol representing a biological replicate (A, B, and D). Data are representative of 2 independent experiments, with n = 4–5 biological replicates/group (D), or pooled samples from 5–8 biological replicates/group (A and B). *P < 0.05, **P < 0.01.