Measurements from alveolar epithelial cells (AECs) at 24 or 48 hours after either PR8 IAV MOI 1 infection or mock infection: (A) MIF released from AECs, (B) green fluorescent protein IAV–positive (GFP-IAV–positive) cells, and (C) total GFP-IAV fluorescence intensity. Statistical differences were determined by unpaired 2-tailed Student’s t test (A–C). The violin plots are closed curves representing data distribution and encapsulate the median, range, and interquartilerange, with each symbol representing a biological replicate (A–C). Data are representative of pooled data, with n = 4 biological replicates/group (A) or 3 biological replicates/group (B and C). *P < 0.05.