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. 2019 Jul 2;17(7):e3000350. doi: 10.1371/journal.pbio.3000350

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© 2019 Bai et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A) Schematic for ICAHCI-mediated functional mutagenic screening in bone development in mice via CRISPR/Cas9. (B) Schematic for comparison of ICAHCI and zygote injection/electroporation mediated genetic screening in mice via CRISPR/Cas9. Cas9, CRISPR-associated protein 9; CEBPβ, CCAAT/enhancer binding protein beta; CEBPδ, CCAAT/enhancer binding protein delta; CRISPR, clustered regularly interspaced palindromic repeats; DKO-AG-haESC, double knockout androgenetic haploid embryonic stem cell; ICAHCI, intracytoplasmic AG-haESCs injection; IRX5, iroquois homeobox 5; MSC, mesenchymal stem cell; PPARγ, peroxisome proliferator activated receptor γ; sgRNA.