Figure 1.

CB₂ deficiency results in an exaggerated acute inflammatory response in the dorsal air pouch inflammation model. Dorsal air pouches of 8–10-wk-old female WT and CB₂^−/− mice were injected with 100 μg Zymosan and lavaged at the indicated time points. A–D) Absolute numbers of total CD45⁺ cells (A), neutrophils (CD45⁺, Cd115⁻, Ly-6G⁺, Ly-6C^lo) (B), Ly-6C^lo (C), and Ly-6C^hi monocytes (CD45⁺, CD115⁺) (D) were determined by flow cytometry. E–H) Blood was also withdrawn from the same animals and numbers of total CD45⁺ cells (E), neutrophils (F), Ly-6C^lo (G), and Ly-6C^hi (H) monocytes per milliliter of blood were determined by flow cytometry. Data are means ± sem (n = 5–11 animals/group). Statistical analysis was conducted by 2-way ANOVA with Sidak’s multiple comparisons correction. **P < 0.01, ***P < 0.001.