Figure 4.

Lack of CB₂ in hematopoietic cells is responsible for the increased neutrophil recruitment to the dorsal air pouches of CB₂^−/− animals. WT and CB₂^−/− bone marrow chimeric female mice (14–16 wk old) containing an equal mix of CD45.1⁺ (WT) and CD45.2⁺ (CB₂^−/−) myeloid cells were used in the dorsal air pouch inflammation model. A–G) Dorsal air pouches were lavaged 6 h after 100 μg Zymosan injection, and absolute numbers of total CD45⁺ cells (A) as well as representative dot plots of CD45⁺CD11b⁺ cells (B, E), neutrophils (C, F), and monocytes (D, G) were determined by flow cytometry from WT and CB₂^−/− recipient animals, respectively. H–K) Absolute numbers of CD45.1⁺WT and CD45.2⁺CB₂^−/− neutrophils (H) and Ly-6C^hi monocytes (I) recruited to the dorsal air pouches of WT and CB₂^−/− recipient mice. Blood was also withdrawn from the same animals, and numbers of CD45.1⁺WT and CD45.2⁺CB₂^−/− neutrophils (J) and Ly-6C^hi monocytes (K) per milliliter of blood were determined by flow cytometry. NS, not significant. Data are means + sem (n = 9–11 animals/group). Statistical analysis was conducted by 2-way ANOVA with Sidak’s multiple comparisons correction. **P < 0.01, ***P < 0.001.