Figure 5.

CB₂^−/− neutrophils from dorsal air pouches display higher expression of migration-related molecules. Dorsal air pouches of 8–10-wk-old female WT and CB₂^−/− mice were injected with 100 μg Zymosan and lavaged 6 h later. Recruited neutrophils were purified with negative selection, and their transcriptome was profiled with the nCounter Gene Expression Kit. A) 36 genes were differentially expressed (DE) in CB₂^−/− neutrophils. Spearman correlation and average linkage were chosen as the clustering parameters in the heatmap. Each row represents 1 gene, and each column represents neutrophils isolated from an independent WT or CB₂^−/− animal. B–F) List of at least 2-fold up-regulated (B) and down-regulated (C) DE genes in CB₂^−/− neutrophils. Ccr7 (D), Ccl22 (E), and Cxcl10 (F) gene expression was validated by qPCR. Data are means + sem (n = 5 independent neutrophil purifications/genotype). Statistical analysis was conducted by a 1-tailed Student’s t test with Welsh’s correction. *P < 0.05, **P < 0.01, ***P < 0.001,