Figure 6.

CB₂^−/− neutrophils from bone marrow adhere more to ICAM-1 upon stimulation with complement and bacterial products. Bone marrows of 8–10-wk-old female WT and CB₂^−/− mice were harvested, and neutrophils were purified with negative selection. Cells were incubated with vehicle, 10 nM C5a, or 20 nM fMLP for 15 min at 37°C and were then transferred to ICAM-1–precoated 96-well plates to adhere for another 15 min. Adhesion quantification was carried out with the PrestoBlue dye. Data are means + sem (n = 4 independent neutrophil purifications/genotype). Statistical analysis was conducted by 2-way ANOVA with Sidak’s multiple comparisons correction. *P < 0.05, **P < 0.01.