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. 2019 Jun 20;4(12):e127167. doi: 10.1172/jci.insight.127167

Figure 1. Screening of human recombinant mAbs.

Figure 1

Recombinant mAbs were produced from single MuSK multimer-sorted B cells. Binding of these clones to MuSK-expressing cells was determined using a flow cytometry–based antibody-binding assay. Each data point represents the mean ΔMFI of each mAb tested at 1 μg/mL in triplicate. Bars represent the mean of means and error bars the SDs. The mAbs were derived from patients with MuSK MG and healthy donors (HDs): MuSK1 (n = 22), MuSK2a (n = 5), MuSK3 (n = 12), MuSK4 (n = 13), MuSK5 (n = 21), MuSK6 (n = 6), HD1 (n = 10), and HD2 (n = 19). A human recombinant MuSK mAb that we previously produced from single-cell–sorted plasmablasts (indicated with an arrow) was included with those tested from patient MuSK3. Values greater than the mean plus 4 SDs of the HD-derived mAbs (indicated by the horizontal dotted line) were considered positive.