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. 2019 Jul 1;13(1):137–140. doi: 10.1080/19336896.2019.1617623

Figure 1.

Figure 1.

In vitro amplification of PrPCWD in disinfectants-treated soil assessed by PMCAb. Different amounts of the recommended disinfectant concentrations (2 N NaOH, 2% NaClO) were treated to the soil for 1 and 16 h. (A) Western blot analysis after 1-h treatment. After three rounds of amplification, no signals were observed in the 100–200 μL 2 N NaOH-treated soils. In 2% NaClO-treated soils, PrPCWD signals were detected for all amounts (50–200 μL). (B) Western blot analysis after 16-h treatment. After three rounds of amplification, no signals were observed in the 2 N NaOH-treated soils. PrPCWD signals were not detected for only the 500 μL 2% NaClO-treated soils. Molecular mass standards (kDa) are indicated on the left. PC: positive control, 0.01% CWD infected elk brain homogenate; NC: negative control, 0.01% normal elk brain homogenate.