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. 2019 Jul 5;13(1):249–259. doi: 10.1080/19336918.2019.1638690

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The cell cycle progression is not affected by BECN1 expression. (a) H1299 cells were transfected with pCMV-HA or pCMV-HA-BECN1. Forty-eight hours later, cells were harvested and analyzed by flow cytometry (Left panel). The quantification of cell number in each phase of the cell cycle derived from left panel was marked with different colors [red: quiescent phase and the first gap phase (G0/G1 phase), green: DNA synthesis phase (S phase), blue: the second gap phase and mitotic phase (G2/M phase)] (Right panel). (b) The mRNA levels of the cell cycle-related genes were determined by Q-PCR in H1299 cells. Data represent the average of three independent experiments (mean±SD). ***, P≤ 0.001; ns, P >0.05. (c) The protein levels of the cell cycle-related genes were determined by Western blot using indicated antibodies in H1299 cells transfected with BECN1 siRNAs.