Figure 2.

miR-25 modulates cell migration and invasion in NSCLC cells. (a) Expression of miR-25 in H2087, HCC44, Calu-1, H358, and H1993 cells were detected by qRT-PCR, RNU6B served as internal control; (b) H358 cells transfected with pre-miR-25 and pre-miR for 48 h were harvested for detection of miR-25 expression by qRT-PCR, RNU6B served as internal control. (c) Calu-1 cells transfected with anti-miR-25 and anti-miR for 48 h were harvested for detection of miR-25 expression by qRT-PCR, RNU6B served as internal control. H358 cells were transfected with 30 nM pre-miR-25 or pre-miR for 24 h, cell invasion (d) and migration (e) was detected. Calu-1 cells transfected with anti-miR-25 and anti-miR for 24 h, cell invasion (f) and migration (g) were detected. (h) H358 was transfected with 30 nM pre-miR and pre-miR-25, and Calu-1 was transfected with anti-miR and anti-25. Forty-eight hours after transfection, cells were collected for apoptosis analysis by annexin V staining and flow cytometry. (i) H358 was transfected with 30 nM pre-miR and pre-miR-25, and Calu-1 was transfected with anti-miR and anti-miR-25. Twenty-four hours after transfection, cell viability was detected using MTT at days 1, 3, and 5 after seeded; the proliferation curve was generated based on the absorbance and times. Columns, mean for three experiments, bars, S.E.; * p< 0.01.