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. 2019 Jul 6;10(1):271–281. doi: 10.1080/21655979.2019.1632668

Figure 3.

Figure 3.

miR-25 regulates the expression of CDH1. (a) The cell lysates of H2087, HCC44, Calu-1, H358, and H1993 cells were prepared for Western blotting using antibody against CDH1; GAPDH was used as a loading control. (b) Calu-1 cells were transfected with 30 nM anti-miR and anti-miR-25, cells were collected for Western blotting using antibody against human CDH1 and GAPDH was used as a loading control. H358 cells were transfected with 30 nM pre-miR and pre-miR-25, cells were collected for Western blotting using antibody against human CDH1 and GAPDH was used as a loading control. (c) expression of CDH1 in normal tissues, tumor tissues, and tumor-adjacent tissues was examined by IHC.