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. 2019 Jul 6;10(1):271–281. doi: 10.1080/21655979.2019.1632668

Figure 4.

Figure 4.

CDH1 plays a critical role in miR-25-mediated invasion and migration in NSCLC cells. (a) H358 cells were co-transfected with pre-miR or pre-miR-25 and pCMV6 or pCMV6-CDH1 for 24 h, Calu-1 cells were co-transfected with anti-miR or anti-miR-25 and control siRNA or CDH1 siRNA for 24 h, expression of CDH1 was detected by Western blot assay. (b) H358 cells were co-transfected with pre-miR or pre-miR-25 and pCMV6 or pCMV6-CDH1 for 24 h, cell invasion and cell migration were detected. (c) Calu-1 cells were co-transfected with anti-miR or anti-miR-25 and control siRNA or CDH1 siRNA for 24 h, cell invasion and cell migration were detected. Cell lysates were prepared for Western blotting using antibody against CDH1 after transfection. *p < 0.05.