Figure 2.

HPLC analysis of cell extracts from sensitized and desensitized cells identifies the pigment as staphyloxanthin (STXN). Ethanol extracts from control, farnesol-sensitized, and desensitized cells were monitored at 450 nm. Based on the resultant chromatograms, a peak with an average retention time of 15.033 min, consistent with that reported for STXN, was detected in extracts from control cells but not from farnesol-sensitized cells. In contrast, STXN peaks similar to those in control cells were detected in the farnesol-desensitized cells, indicating restoration of the pigment upon removal of farnesol supplementation from the media. HPLC analyses were obtained from three independent, biological replicates; representative chromatograms and images of extracts are shown.