Figure 2.
Shear sensitivity of voltage-dependent gating kinetics of G615E NaV1.5 compared to WT NaV1.5. (a-f), Time constants of activation (a, d; τA) and two inactivation components (b-c, e-f) from wild-type (a-c, WT) or G615E (d-f) NaV1.5 currents, before (●) or during shear stress (○) by flow of extracellular solution at 10 mL/min [n = 12 cells each; *P < 0.05 shear stress vs. baseline controls and P < 0.05 interaction between voltage and shear (τA) or between genotype and shear (τF, τS) by a three-way ANOVA with Tukey post-test; †P< 0.05 to WT by a two-tailed unpaired t-test]. (g-i), Average change in time constants of activation (g) and two inactivation components (h-i) of WT (black) or G615E (red) NaV1.5 currents at −30 mV (n = 12 cells each; *P < 0.05% to 0% by a two-tailed one-sample t-test; †P < 0.05 to WT by a two-tailed unpaired t-test).