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. 2019 Jul 11;4(13):e128269. doi: 10.1172/jci.insight.128269

Figure 2. Ube2o ablation protects mice against diet-induced obesity and metabolic syndrome.

Figure 2

(A) Body weight increase of Ube2o+/+ and Ube2o–/– mice fed an HFD for 21 weeks. Ube2o+/+ n = 7, Ube2o–/– n = 9. (B) Food intake (grams per day) in Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. Ube2o+/+ n = 6, Ube2o–/– n = 8. (C) Fat mass of Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. Ube2o+/+ n = 5, Ube2o–/– n = 9. (D) Adipose depot weight of Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. SAT, subcutaneous adipose tissue; VAT, visceral adipose tissue; BAT, brown adipose tissue. Ube2o+/+ n = 6, Ube2o–/– n = 9. (E) H&E-stained sections of SAT in Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. Scale bars: 75 μm. (F) Plasma levels of total cholesterol (TC) (mg/dL), free fatty acid (FFA; mEq/L), HDL (mg/dL), LDL (mg/dL) in Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. n = 6. (G) Liver weight of Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. Ube2o+/+ n = 6, Ube2o–/– n = 9. (H) Hepatic triglyceride (TG) level per gram of liver in Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. n = 6. (I) H&E-stained sections of liver in Ube2o+/+ and Ube2o–/– mice on an HFD for 28 weeks. Scale bars: 75 μm. (J) Oxygen consumption (VO2) per kg lean mass of Ube2o+/+ and Ube2o–/– mice on an HFD for 20 weeks was determined in metabolic chambers. Ube2o+/+ n = 6, Ube2o–/– n = 9. (K) Respiratory exchange ratio (RER; VCO2/VO2) of Ube2o+/+ and Ube2o–/– mice on an HFD for 20 weeks was determined in metabolic chambers. Ube2o+/+ n = 6, Ube2o–/– n = 9. (L) Energy expenditure during day and night per kg of lean mass of Ube2o+/+ and Ube2o–/– mice on an HFD for 20 weeks was determined in metabolic chambers. Ube2o+/+ n = 6, Ube2o–/– n = 9. (M) Home cage locomotor activity during both the light and dark phases of Ube2o+/+ and Ube2o–/– mice on an HFD for 20 weeks was determined by using an automated combined indirect calorimetry system. Ube2o+/+ n = 5, Ube2o–/– n = 8. (N) Rectal temperature of Ube2o+/+ and Ube2o–/– mice on an HFD for 12 weeks. Ube2o+/+ n = 8, Ube2o–/– n = 9. Error bars represent ±SEM. P value was determined by Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001).