Figure 8. A high-salt diet increases inflammation in the colon and Peyer’s patches of mice.

(A) Immunohistochemistry images showing distribution of T cells and monocyte/macrophages in the entire gut of a high-salt diet–fed mouse. Scale bar: 100 μm. (B) Representative immunohistochemistry images showing increased infiltration of T cells and monocyte/macrophages in the colon of high-salt diet–fed mice compared with normal salt diet–fed mice. Scale bar: 100 μm. (C) Representative images showing increased infiltration of T cells and monocyte/macrophages in the Peyer’s patches of high-salt diet–fed mice compared with normal salt diet–fed mice. Scale bar: 100 μm. (D) Flow cytometry gating strategy to identify effector memory T cell subtypes. (E) Effect of high-salt feeding on total CD45⁺ cells, CD3⁺ T cells, CD4⁺ T cells, CD8⁺ T cells, and effector memory CD4⁺ and CD8⁺ T cells in the colon. (F) Effect of high-salt feeding on total CD45⁺ cells, CD3⁺ T cells, CD4⁺ T cells, CD8⁺ T cells, and effector memory CD4⁺ and CD8⁺ T cells in the Peyer’s patches (percentages and numbers are shown; *P < 0.05, **P < 0.001 using 2-tailed unpaired Student’s t tests).