Mice were fed a normal salt diet (black) or a high-salt diet, 8% NaCl (red), for 3 weeks. Osmotic minipumps were implanted at 3 weeks to deliver a subcutaneous low dose of angiotensin II (140 mg/kg/h) for 2 weeks. (A) Experimental strategy. Systolic (B), diastolic (C), mean arterial blood pressure (D), and heart rate (E) were monitored using radiotelemetry (n = 5 normal salt and 6 high salt; *P < 0.05, **P < 0.001, ****P < 0.0001 high salt versus normal salt control, using 2-way repeated-measures ANOVA). D, day; N, night.