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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Methods Mol Biol. 2019;1891:165–177. doi: 10.1007/978-1-4939-8904-1_12
10× Taq buffer 1.0 μL
5 mM dNTP mix 1.0 μL
0.5 unit/μL Taq DNA polymerase 1.5 μL
25 mM MgCl2 1.5 μL
50 μM 5′ primer (Table 1) 0.1 μL
50 μM 3′ primer (Table 1) 0.1 μL
Water 0.8 μL
Template DNA (20× diluted) 4.0 μL