Table 1.

Primers used for genotyping of different BMP receptors genetically modified mice

	Ref	Modified gene	Primers	Primer ratios	PCR products	Annealing
Global knockout mutation
Fig. 1a	[6]	Alk2 null	A2–5 5′-ATGCTAGACCTGGGCAGCCATA-3′ PGK-Mot 5′-CGTGTGTAAGGTGTAGGTG GCC-3′	A2–5:PGK-Mot: A2–3 = 2:1:1	195 bp (Alk2 null)	65 °C, 40 cycles
			A2–5 5′-ATGCTAGACCTGGGCAGCCATA-3′ A2–3 5′-CATGCTAGCAGCTCGGAGAAAC-3′		371 bp (wild type)
Fig. 1b	[18]	Alk3 null	fx3 5′-AGACTGCCTTGGGAAAAGCGC-3′ fx5 3′-GGACTATGGACACACAATGGC-3′	fx3:fx5:fx0 = 1:2:1	190 bp (Alk3 null)	65 °C, 40 cycles
			fx5 5′-GGACTATGGACACACAATGGC-3′ fx0 5′-CTCTGAATTTCTAGTCCACATCTGC-3′		280 bp (wild type)
Fig. 1c	[7]	Alk6 null	Alk6-I 5′-TGGTGAGTGGTTACAACAAGATC AGCA-3′	Neo-Al2: Alk6-I: Alk6-E = 1:2:1	300 bp (Alk6 null)	65 °C, 40 cycles
			Neo-Al2 5′-GAAAGAACCAGCTGGGGCTC GAG-3′
			Alk6-I 5′-TGGTGAGTGGTTACAACAAGATC AGCA-3′		350 bp (wild type)
			Alk6-E 5′-CTCGGCCCAAGATCCTACGTTG-3′
Fig. 1d	[14]	Bmpr2 null	A 5′-GCTAAAGCGCATGCTCCAGACTGCC TT-3′	Primer A:B:C = 2:1:1	260 bp (Bmpr2 null)
			C 5′-AGGTTGGCCTGGAACCTGAGGAAATC-3′
			A 5′-GCTAAAGCGCATGCTCCAGACTGCC TTG-3′		200 bp (wild type)
			B 5′-TCACAGCATGAACATGATGGAGGCGG-3′
Fig. 1e	[16, 32]	Acvr2a null	A 5′-TGGGAAGACAATAGCAGGCATGC-3′	1:1:1:1	900 bp (Acvr2A null)	70 °C, 30 cycles
			B 5′-GCAGAGTGTGACCCGTACCCAC-3′
			C 5′-GTTGGTACCCGGAGGTATATGGC-3′		140 bp (wild type)
			D 5′-CCCTTACCATCTGCAGCAGTGCA-3′
		Acvr2b null	A 5′-ATGAACTGCAGGACGAGGCAGCG-3′	1:1:1:1	600 bp (Acvr2B null)
			B 5′-GGCGATAGAAGGCGATGCGCTG-3′
			C 5′-CCGACAGCCCCCACCCTGCTCA-3′		241 bp (wild type)
			D 5′-GGCCCACCAGAGGGGATGGGGG-3′
Conditional knockout mutation
Fig. 2a	[29]	Alk2 flox	Alk217F 5′-CCCCCATTGAAGGTTTAGAG AGAC-3′	Alk217F: lk217R2 = 1:1	160 + 90 bp (flox) (see Note 4), 250 bp (wild type)	65 °C, 40 cycles
			Alk217R2 3′-CTAAGAGCCATGACAGA GGTTG-3′
		Alk2-recombined	Alk2RecF 5′-GAATTGCTAGAAGCCCATA GGC-3′	Alk2RecF:Alk2WTR: Alk217F = 1:2:1	625 bp (recombined)	60 °C, 40 cycles
			Alk2WTR 5′-TGAGATTGTTCTAGCACTGC CC-3′
			Alk217F 5′-CCCCCATTGAAGGTTTAGAGA GAC-3′		530 bp (wild type)
			Alk2WTR 5′-TGAGATTGTTCTAGCACTGC CC-3′
Fig. 2b	[8]	Alk3 flox	fx2 5′-GCAGCTGCTGCTGCAGCCTCC-3′	fx2:fx4 = 1:1	230 bp (flox)	65 °C, 40 cycles
			fx4 3′-TGGCTACAATTTGTCTCATGC-3′		150 bp (wild type)
Fig. 2b		Alk3-recombined (see Note 6)	fx1 5′-GGTTTGGATCTTAACCTTAGG-3′	fx1:fx4:BMP2-A: BMP2-B = 1:1:1:1	180 bp (recombined)	55 °C, 40 cycles
			fx4 5′-TGGCTACAATTTGTCTCATGC-3′
			BMP2-A 5′-AGCATGAACCCTCATGTGTT GG-3′		322 bp (wild type)
			BMP2-B 5′-GTGACATTAGGCTGCT GTAGCA-3′
Fig. 2c	[15]	Bmpr2 flox	5F 50-GGCAGACTCTGACTTTGACGCTAG-3′		315 bp (2loxP and 3loxP)	60 °C, 40 cycles
			C 5′-TTATTGTAAGTACACTGTTGCTGTC-3′
			A 5′-CACACCAGCCTTATACTCTAG ATAC-3′		260 bp (wild type)
			6R 5′-CACATATCTGTTATGAAACTTGAG-3′		350 bp (2loxP)
			A 5′-CACACCAGCCTTATACTCTAG ATAC-3′		270 bp (wild type)
			C 5′-TTATTGTAAGTACACTGTTGCTGTC-3′		500 bp (1loxP)
Conditional constitutively active transgenic line
Before recombination
Fig. 2d	[12]	caAlk	TF41 5′-GTGCTGGTTATTGTGCTGTCTC-3′	TF41:TF61:LbnFR1: LbnRev3 = 1:1:1:1	580 bp (caAlk transgene)	65 °C, 40 cycles
			TF61 5′-GACGACAGTATCGGCCTCAGGAA-3′
			LbnFR1 5′-GAGGACGCAGTCCAGTACCT-3′		334 bp (Internal control)
			LbnRev3 5′-TAGCCTCTGCCTCACGCCCT GC-3′
After recombination
Fig. 2d	[11]	caAlk2	TF41 (common) 5′-GTGCTGGTTATTGTG CTGTCTC-3′	TF41:TF9 = 1:1	750 bp (caAlk2 transgene)	65 °C, 40 cycles
			TF9 (Alk2) 5′-CGAACACTACAGAGAGAAT AATG-3′
	[12]	caAlk3	TF41 (common) 5′-GTGCTGGTTATTGTGC TGTCTC-3′	TF41:TF17 = 1:1	300 bp (caAlk3 transgene)
			TF17 (Alk3) 5′-CGGCGTAGCTGGGCTTTT GGA-3′
		caAlk6	TF41 (common) 5′-GTGCTGGTTATTGTGC TGTCTC-3′	TF41:TF25 = 1:1	300 bp (caAlk6 transgene)
			TF25 (Alk6) 5′-GACATCCAGAGGTGACAA CAG-3′