Table 2.
TaqMan primers used for genomic real-time PCR of different BMP receptors genetically modified mice
| Ref | Modified gene | Primers |
|---|---|---|
| Conditional knockout mutation | ||
| [31] |
Alk2 (exon 7) (see Note 5) |
AIKAL5S_F 5′-CTCACTACTCTGGATACGGTTAGCT _3′, AIKAL5S_R 5′-GGGTCCCAAATATCTCTATGTGCAA-3′, AIKAL5S_M FAM 5′-CTATGGACAGTACAATCCG-3′ |
| [30, 31] |
Alk3 (exon 4) (see Note 5) |
AI89LJ8_F 5′-GACCAGAAGAAGCCAGAAAATGGA-3′, AI89LJ8_R 5′-TGTCCTGAGCAATAGCACTTTAAGAA-3′, AI89LJ8_M FAM 5′-CCTCTGGTGCTA AAGTC-3′ |
| Conditional constitutively active transgenic line | ||
|
Egfp (see Note 5) |
5′-GAGCGCACCATCTTCTTCAAG-3′, 5′-TGTCGCCCTCGAACTTCAC-3′, FAM 5′-ACGACGGCAACTACA-3′ |
|