KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial, Archaeal and Virus Strains | ||
| Agrobacterium tumefaciens 3101 (Rhizobium radiobacter) | Savithramma Dinesh-Kumar | CJW501 (At) |
| Anaerostipes sp. | Andrew Goodman | N/A (As) |
|
Asticcacaulis excentricus ATCC 15261 |
Jeanne S. Poindexter | CJW960 (Ae) |
| Bacillus megaterium ATCC 14581 | ATCC Bacteriology Collection | CJW5752 (Bm) |
| Bacillus subtilis subsp. subtilis str. NCIB 3610 | Wade Winkler | CJW5495 (Bs) |
| Bacteroides ovatus | Andrew Goodman | N/A (Bt) |
| Bacteroides thetaiotaomicron | Andrew Goodman | N/A (Bo) |
| Bacteroides xylanisolvens | Andrew Goodman | N/A (Bx) |
| Brevundimonas bacteroides | Pamela Brown | CJW5566 (Brb) |
| Brevundimonas diminuta | Pamela Brown | CJW5571 (Brd) |
| Brevundimonas subvibrioides | Pamela Brown | CJW5565 (Brs) |
| Burkholderia thailandensis E264 | Peter Greenberg | CJW5484 (But) |
| Caulobacter crescentus CB15N | (Evinger and Agabian, 1977) | (Cc) |
| C. crescentus CB15N rodZ::Himar1 | (Alyahya et al., 2009) | CJW1842 |
| C. crescentus CB15N popZ::omega | (Ebersbach et al., 2008) | CJW2238 |
| C. crescentus CB15N ftsZ::Pxyl-ftsZ rplA::pL1-GFPC-1 | (Montero Llopis et al., 2012) | CJW3821 |
| C. crescentus CB15N rplA::pL1-dendra2 | (Lim et al., 2014) | CJW5156 |
| C. crescentus CB15N hfq::tet | (Irnov et al., 2017) | CJW5477 |
| C. crescentus CB15N hu2::pCHYC2-hu2' | (Arias-Cartin et al., 2017) | CJW5806 |
| C. crescentus CB15N hu2::pCHYC2-hu2' dnaN::pCFPC1-dnaNend' | (Arias-Cartin et al., 2017) | CJW5969 |
| C. crescentus CB15N xylX::pXyl-egfp-μNS-kan | This work | CJW6917 |
| Cellulophaga algicola IC166T | Mark McBride | DSM14237 (Ca) |
| Chromobacterium violaceum | Jo Handelsman | N/A (Cv) |
| Chryseobacterium indologenes | Jo Handelsman | CJW4422 (Ci) |
| Clostridium boltae | Andrew Goodman | N/A (Cb) |
| Clostridium hathewayi | Andrew Goodman | N/A (Ch) |
| Collinsella aerofaciens | Andrew Goodman | N/A (Coa) |
| Cytophaga hutchinsonii ATCC 33406 (glucose-adapted) | Mark McBride (Zhu and McBride, 2014) |
CJW5842 (Cyh) |
| Escherichia coli MG1655 | (Guyer et al., 1981) | (Ec) |
| E. coli MG1655 hupB::hupB-cfp | This work | CJW4656 |
| E. coli MG1655 rplA::rplA-gfp | This work | CJW4677 |
| E. coli BW25113 hupA::hupA-mcherry | (Paintdakhi et al., 2016) | CJW5556 |
| E. coli MG1655 seqA::seqA-mcherry ftsZ::ftsZ-venusSW | This work | CJW6324 |
| E. coli MG1655 dnaC2 hupA::hupA-mcherry | This work | CJW6370 |
| E. coli MG1655 PlacOAYZ::Plac-gfp-μNS hupA::hupA-mcherry | This work | CJW6723 |
| E. coli BW25993 rplA::rplA-meos2 | (Sanamrad et al., 2014) | CJW6768 |
| E. coli BW25993 rpsB::rpsB-meos2 | (Sanamrad et al., 2014) | CJW6769 |
| E. coli MG1655 rplA::rplA-msfgfp | This work | CJW7020 |
| E. coli MG1655 rpsB::rpsB-msfgfp | This work | CJW7021 |
| Flavobacterium johnsoniae ATCC 17061T | Mark McBride | CJW5841 (Fj) |
| Haloferax volcanii DS2 | Ethan Garner | N/A (Hv) |
| Hirschia rosenbergii | Pamela Brown | CJW5574 (Hr) |
| Janthinobacterium lividum | Jo Handelsman | CJW4423 (Jl) |
| Myxococcus xanthus DZF1 | David Zusman | CJW5485 (Mx) |
| Lactobacillus reuteri | Andrew Goodman | N/A (Lr) |
| Lysinibacillus sphaericus ATCC 4525 | ATCC Bacteriology Collection | CJW5751 (Ls) |
| Paenibacillus polymyxa ATCC 7070 | ATCC Bacteriology Collection | CJW5750 (Pp) |
| Parabacteroides distasonis | Andrew Goodman | N/A (Pd) |
| Providencia alcalifaciens | Andrew Goodman | N/A (Pa) |
| Pseudomonas syringae pv. syringae B728a | Steven Lindow | CJW410 (Ps) |
| Rhizobium leguminosarum bv. trifolii R200 | Nora Ausmees | CJW355 (Rl) |
| Roseburia intestinalis | Andrew Goodman | N/A (Ri) |
| Sinorhizobium meliloti 1021 | Jacques Batut | CJW356 (Sm) |
| Vibrio fischeri ES114 | Eric Stabb | CJW5630 (Vf) |
| Vibrio harveyi BB120 | Bonnie Bassler | CJW5482 (Vh) |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Agarose | AmericBio | Cat#AB00972–00500 |
| Cephalexin hydrate | Sigma Aldrich | Cat#C4895 |
| 4',6-Diamidine-2'-phenylindole dihydrochloride (DAPI) fluorescent dye | Thermo Fisher Scientific | Cat#D1306 |
| DpnI restriction enzyme | New England Biolabs | Cat#R0176S |
| Hoechst 33342 fluorescent dye | Thermo Fisher Scientific | Cat#H3570 |
| KpnI restriction enzyme | New England Biolabs | Cat#R0142S |
| NheI restriction enzyme | New England Biolabs | Cat#R0131S |
| Phusion high-fidelity polymerase | New England Biolabs | Cat#M0530S |
| SYBR Green fluorescent dye | Thermo Fisher Scientific | Cat#S7564 |
| T5 exonuclease | New England Biolabs | Cat#M0363S |
| Taq DNA ligase | New England Biolabs | Cat#M0208L |
| Oligonucleotides | ||
| Primers for strain construction | This work, Integrated DNA Technologies |
See Table S3 |
| Recombinant DNA | ||
| pKD13-ftsZ-venusSW-lpxC-FRT-kan-FRT-secM' | This work | N/A |
| pKD3-mCherry | This work | N/A |
| Software and Algorithms | ||
| ImageJ | (Collins, 2007) | https://imagej.nih.gov/ij/ |
| MATLAB | Mathworks | https://www.mathworks.com/ |
| Oufti | (Paintdakhi et al., 2016) | https://oufti.org/ |