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Akt/GSK-3β/β-catenin signaling pathway was activated by NBP in BMECs. (A) Protein level of p-Akt, Akt, p-GSK-3β, GSK-3β, β-catenin proteins detected by Western blotting. (B) Densitometric analysis of protein expression (n = 6). ∗P < 0.01 vs. the control group; †P < 0.05, ‡P < 0.01 vs. the hypoxia group. Akt: Protein kinase B; BMECs: brain microvascular endothelial cells; GSK-3β: Glycogen synthase kinase 3β; NBP: DL-3-n-butylphthalide.
