Skip to main content
. 2019 Jun 5;132(11):1336–1343. doi: 10.1097/CM9.0000000000000187

Figure 2.

Figure 2

Effect of vimentin suppression by siRNA on cell survival assessed by TUNEL assay. THP-1 cells were transfected with vimentin-specific or scramble siRNA followed by exposure to LPS (10 μg/mL), and apoptosis was assessed by TUNEL assay as described in the methods. (A–D) Representative images of TUNEL assay. Green: positive TUNEL staining; blue: nuclei staining with DAPI. (E) Average of three separate TUNEL assay results. Vertical axis: TUNEL positivity (%); horizontal axis: cells transfected with control or vimentin-specific siRNA. Open bar: cells cultured in SF-DMEM only; closed bar: cells treated with LPS. ∗P < 0.05. LPS: Lipopolysaccharide; SF-DMEM: Serum-free Dulbecco modified Eagle media; TUNEL: TdT-mediated dUTP Nick-End Labeling.