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. 2019 Jun 5;132(11):1336–1343. doi: 10.1097/CM9.0000000000000187

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Copyright © 2019 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0

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Effect of vimentin over-expression on cell survival assessed by TUNEL assay. THP-1 cells were transfected with a plasmid containing either a negative control vector or vimentin gene followed by exposure to LPS (10 μg/mL), and apoptosis was assessed by TUNEL assay as described in the methods. (A–D) Representative images of TUNEL assay. Green: positive TUNEL staining; blue: nuclei staining with DAPI. (E) average of three separate TUNEL assay results. Vertical axis: TUNEL positivity (%); horizontal axis: cells transfected with control or vimentin-specific siRNA. Open bar: cells cultured in SF-DMEM only; closed bar: cells treated with LPS. ^∗P < 0.05. LPS: Lipopolysaccharide; SF-DMEM: Serum-free Dulbecco modified Eagle media; TUNEL: TdT-mediated dUTP Nick-End Labeling.