Figure 7.
Effect of vimentin suppression or over-expression on cytokine release. Vimentin was either suppressed by siRNA or over-expressed by transfecting a plasmid containing vimentin promoter followed by exposure to LPS (10 μg/mL), and cytokines were quantified by ELISA as described in the methods. (A) IL-6. (B) TNF-α. (C) IL-10. Vertical axis: cytokine concentration (pg/day/105 cells); horizontal axis: cells transfected with control-siRNA, vimentin-specific siRNA or a plasmid containing vimentin promoter. Open bar: cells cultured in SF-DMEM only; closed bar: cells treated with LPS. Data presented were an average of three separate experiments. ∗P < 0.05. IL: Interleukin; LPS: Lipopolysaccharide; SF-DMEM: Serum-free Dulbecco modified Eagle media.