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. 2019 Jun 13;8:e45545. doi: 10.7554/eLife.45545

Figure 5. Neurons in Gfap Smo CKO mice exhibit a significant increase in excitability and synaptic transmission.

(A) Example traces of action potentials from layer V pyramidal neurons in WT and Gfap Smo CKO mice at P21. Samples of action potential spikes (dashed lines, lower panel) describe AP threshold, AP peak amplitude, and AP 1⁄2 width of pyramidal neurons. (B) Line graph displays relationship between action potential spike numbers (y-axis) and current injection; Gfap Smo CKO neurons exhibit an increase in AP spikes (>500 pA) compared to WT neurons. Statistical significance was assessed by two-way ANOVA with Sidak’s test for multiple comparisons and stated as Gfap Smo CKO compared to WT at a given current (n = 11 and 7 cells from WT and Gfap Smo CKO, respectively). (C–F) Bar graphs describe an increase in total AP spike numbers (C), reduction in AP threshold (mV) (D), reduction in AP 1/2 width (E), and increase in AP peak amplitude (pA) (F) in Gfap Smo CKO neurons. (G) Example traces of sEPSCs from layer V pyramidal neurons recorded in the presence of picrotoxin from WT and Gfap Smo CKO mice at P21. (H–I) Summary graphs of sEPSC frequency and amplitude (n = 14 and 11 cells from WT and Gfap Smo CKO animals, respectively). (J) Example traces of mEPSCs from layer V pyramidal neurons recorded in the presence of picrotoxin and TTX. (K–L) Summary graphs of mEPSC frequency and amplitude (n = 16 cells per genotype). Gfap Smo CKO neurons exhibit increases in both frequency and amplitude in sEPSCs and mEPSCs recordings. Statistical significance was assessed by unpaired Student’s t-test. For each graph, data points represent individual cells. All data are from at least three animals per genotype.

Figure 5—source data 1. Physiological properties of neurons and response to current injections.
elife-45545-fig5-data1.xlsx (19KB, xlsx)
DOI: 10.7554/eLife.45545.021
Figure 5—source data 2. Measurements of spontaneous and miniature EPSCs.
elife-45545-fig5-data2.xlsx (16.7KB, xlsx)
DOI: 10.7554/eLife.45545.022

Figure 5.

Figure 5—figure supplement 1. Membrane properties of Gfap Smo CKO layer V pyramidal neurons.

Figure 5—figure supplement 1.

There is a significant decrease in input resistance (MΩ) in Gfap Smo CKO neurons compared to WT neurons. No significant differences were observed in resting membrane potential (mV), rheobase (pA), and tau (ms) in Gfap Smo CKO neurons. Statistical significance was assessed by Mann-Whitney U test (input resistance and tau) and unpaired Student’s t-test (resting membrane potential).