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. 2019 Jun 13;8:e45545. doi: 10.7554/eLife.45545

Key resources table.

Reagent type
(species) or
resource
Designation Source
or reference
Identifiers Additional
information
Genetic reagent (M. musculus) Gli1CreER/+ The Jackson Laboratory; Ahn and Joyner, 2005 stock no. 7913 from Alexandra Joyner, MSKCC
Genetic reagent (M. musculus) Ai14 (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) The Jackson Laboratory; Madisen et al., 2010 stock no.7914
Genetic reagent (M. musculus) Gfap-Cre, line 73.12 The Jackson Laboratory; Garcia et al., 2004 stock no. 12886 from Michael Sofroniew, UCLA
Genetic reagent (M. musculus) Smofl/fl The Jackson Laboratory; Long et al., 2001 stock no.4526
Genetic reagent (M. musculus) CamKinaseIIα-Cre (CamKIIα-Cre) The Jackson Laboratory; Tsien et al., 1996 stock no. 5359
Genetic reagent (M. musculus) Thy1-GFPm The Jackson Laboratory; Feng et al., 2000 stock no. 7788
Chemical compound, drug tamoxifen Sigma catalog no. T5648
Antibody Polyclonal rabbit anti-GFP Millipore catalog no. AB3080 1:5000 (brightfield); 1:1000 (fluorescence)
Antibody Polyclonal rabbit anti-Kir4.1 Millipore catalog no. AB5818 1:1000 (fluorescence)
Antibody Polyclonal rabbit anti-Gfap Agilent Dako catalog no. Z033429-2; 1:20,000 (brightfield)
Antibody Monoclonal mouse anti-NeuN Millipore catalog no. MAB377 1:1000 (fluorescence)
Antibody Polyclonal rabbit anti-RFP MBL International catalog no. PM005 1:500 (fluorescence)
Antibody Monoclonal rat anti-Ctip2 Abcam catalog no. ab18465 1:2000 (fluroescence0
Antibody Polyclonal sheep anti-CAII Bio-Rad catalog no. AHP206 1:2000 (fluorescence)
Antibody Polyclonal rabbit anti-S100B Agilent Dako catalog no. Z0311 1:1000 (fluorescence)
Antibody Polyclonal rabbit anti-Olig2 Millipore catalog no. AB9610 1:1000 (fluorescence)
Software, algorithm computer assisted tracing of dendrites and spines; Sholl analysis of astrocytes Neurolucida, MBF Biociences Williston, VT
Software, algorithm MATLAB-based custom-written code for longitudinal analysis of spine turnover This paper Figure 3—source code 1