Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (M. musculus) | Gli1CreER/+ | The Jackson Laboratory; Ahn and Joyner, 2005 | stock no. 7913 | from Alexandra Joyner, MSKCC |
Genetic reagent (M. musculus) | Ai14 (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) | The Jackson Laboratory; Madisen et al., 2010 | stock no.7914 | |
Genetic reagent (M. musculus) | Gfap-Cre, line 73.12 | The Jackson Laboratory; Garcia et al., 2004 | stock no. 12886 | from Michael Sofroniew, UCLA |
Genetic reagent (M. musculus) | Smofl/fl | The Jackson Laboratory; Long et al., 2001 | stock no.4526 | |
Genetic reagent (M. musculus) | CamKinaseIIα-Cre (CamKIIα-Cre) | The Jackson Laboratory; Tsien et al., 1996 | stock no. 5359 | |
Genetic reagent (M. musculus) | Thy1-GFPm | The Jackson Laboratory; Feng et al., 2000 | stock no. 7788 | |
Chemical compound, drug | tamoxifen | Sigma | catalog no. T5648 | |
Antibody | Polyclonal rabbit anti-GFP | Millipore | catalog no. AB3080 | 1:5000 (brightfield); 1:1000 (fluorescence) |
Antibody | Polyclonal rabbit anti-Kir4.1 | Millipore | catalog no. AB5818 | 1:1000 (fluorescence) |
Antibody | Polyclonal rabbit anti-Gfap | Agilent Dako | catalog no. Z033429-2; | 1:20,000 (brightfield) |
Antibody | Monoclonal mouse anti-NeuN | Millipore | catalog no. MAB377 | 1:1000 (fluorescence) |
Antibody | Polyclonal rabbit anti-RFP | MBL International | catalog no. PM005 | 1:500 (fluorescence) |
Antibody | Monoclonal rat anti-Ctip2 | Abcam | catalog no. ab18465 | 1:2000 (fluroescence0 |
Antibody | Polyclonal sheep anti-CAII | Bio-Rad | catalog no. AHP206 | 1:2000 (fluorescence) |
Antibody | Polyclonal rabbit anti-S100B | Agilent Dako | catalog no. Z0311 | 1:1000 (fluorescence) |
Antibody | Polyclonal rabbit anti-Olig2 | Millipore | catalog no. AB9610 | 1:1000 (fluorescence) |
Software, algorithm | computer assisted tracing of dendrites and spines; Sholl analysis of astrocytes | Neurolucida, MBF Biociences | Williston, VT | |
Software, algorithm | MATLAB-based custom-written code for longitudinal analysis of spine turnover | This paper | Figure 3—source code 1 |