Table 1. Properties of VIP-INs from Scn1a+/- and WT littermates Listed p-values indicate the result of a Kruskal Wallis test for an effect by group.
Pairwise comparisons are with Dunn's test. All comparisons are made considering the average of each mouse as n = 1.* p<0.05 vs. age and subtype matched wild-type; **p<0.01; ***p<0.001. † p<0.05 between CA and IS subtypes (consistent between WT and Scn1a+/-). Data for each cell are reported in Figure 3—source data 1.
| Subtype | CA | IS | p-value | ||||
|---|---|---|---|---|---|---|---|
| Genotype | WT | Scn1a+/- | WT | Scn1a+/- | (group) | ||
| n mice (cells) | 30(71) | 20(43) | 32(78) | 22(66) | |||
| Age (days) | 33.7 ± 1.9 | 33.5 ± 2.6 | 33.4 ± 2.0 | 34.0 ± 2.4 | 0.8 | ||
| Vm (mV) | −66.3 ± 1.0 | −65.2 ± 1.0 | −65.6 ± 0.8 | −64.0 ± 0.6 | 0.3 | ||
| Rm (MΩ) | 355 ± 16 | 391 ± 21 | 344 ± 17 | 314 ± 11 | 0.2 | ||
| Time Constant | 9.17 ± 0.9 | 9.98 ± 1.0 | 10.7 ± 1.5 | 10.1 ± 0.9 | 0.2 | ||
| Rheobase (pA) | 48.3 ± 3.2 | 45.4 ± 4.1 | 53.5 ± 3.6 | 57.7 ± 4.2 | 0.2 | ||
| AP Threshold (mV) | −41.8 ± 0.5 | −41.5 ± 0.5 | −41.2 ± 0.5 | * | −39.1 ± 0.4 | 0.01 | |
| AP Rise Time (ms) | 0.45 ± 0.01 | 0.46 ± 0.01 | 0.44 ± 0.01 | 0.44 ± 0.01 | 0.1 | ||
| Max Rise Slope (mV/ms) | 418 ± 14 | 417 ± 13 | 427 ± 17 | 371 ± 15 | 0.1 | ||
| AP Halfwidth (ms) | 0.55 ± 0.02 | 0.54 ± 0.02 | † | 0.50 ± 0.02 | 0.49 ± 0.02 | 0.04 | |
| AP Amplitude (mV) | 79 ± 1.3 | 80 ± 1.2 | 78 ± 1.6 | * | 71 ± 1.8 | 0.004 | |
| AHP Amplitude (mV) | 10.0 ± 0.63 | 10.9 ± 0.55 | 10.6 ± 0.55 | 11.0 ± 0.43 | 0.6 | ||
| AHP time (ms) | 1.75 ± 0.16 | 1.76 ± 0.21 | 1.51 ± 0.14 | 1.41 ± 0.11 | 0.09 | ||
| Sag (percent) | 14.4 ± 1.3 | 16.3 ± 1.5 | † | 23.5 ± 2.6 | 28.0 ± 2.0 | 8 E −5 | |
| APs at Rheobase | 1.38 ± 0.1 | 1.32 ± 0.1 | † | 2.41 ± 0.3 | 2.01 ± 0.2 | 7 E −5 | |
| Instantaneous (Hz) | 237 ± 11 | 235 ± 13 | 245 ± 10 | 209 ± 11 | 0.1 | ||
| Steady-State (Hz) | 81 ± 4 | 81 ± 4 | † | 55 ± 4 | ** | 25 ± 3 | 2 E −12 |
| ISI CoV | 0.30 ± 0.02 | 0.31 ± 0.02 | † | 0.61 ± 0.03 | 0.69 ± 0.04 | 4 E −13 | |