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. Author manuscript; available in PMC: 2020 Jul 12.
Published in final edited form as: Circ Cardiovasc Imaging. 2019 Jul 12;12(7):e009063. doi: 10.1161/CIRCIMAGING.119.009063

Figure 6: Serial detection of increased AAV9-driven NIS expression over time using microSPECT/CT.

Figure 6:

A) Representative coronal and transverse microSPECT/CT images of 99mTcO4− uptake in gastrocnemius muscles on days 3, 7, 10, 14 and 28 following intramuscular injection of AAV9-hNIS in a mouse that underwent unilateral surgical ischemia of the right hind-limb and had PBS delivered into the left hind-limb. Each representative image’s display scale was normalized to the injected dose and body weight and displayed as SUV. B) Time course C) Quantitative analysis of gastrocnemius 99mTcO4− uptake derived from microSPECT/CT images, and D) Correlation between 99mTcO4− uptake values using VOIs generated by the deep learning algorithm compared to ex vivo 99mTcO4− uptake values from tissue well counting. For image quantification, image activity in mCi/cc was corrected for injected dose and animal body weight, and also displayed as, SUV. * = P<0.05 vs. PBS; † = P<0.05 vs. AAV9-hNIS; ‡ P<0.05 vs. day, § P<0.01 vs. day 3, || P=0.0001 vs. day 3; # = P < 0.05 vs. day 7; ** = P< 0.05 vs. day 10 and ‡‡= P<0.05 vs. day 14.