Fig. 4.

Application of limonene and nerolidol to mycorrhizal M. truncatula and A. euteiches zoospores, respectively. (A). Mycorrhizal M. truncatula plants were treated with 1 nM limonene twice per week, whereas control plants remained untreated. After 3 weeks, mycorrhizal colonization was determined by quantification of transcript levels of Riβ-Tub using qRT-PCR. (B). Number of vital A. euteiches zoospores treated for 15 min with different concentrations of nerolidol. Bars indicate means ± SDs; n = 9 in A, and n = 3 in B. Student’s t-test revealed no significant differences among the treatments.