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CRISPR/Cas9-mediated targeting of Rev-erbα and Rev-erbβ in mES cells. (a) Schematic of Rev-erbα and Rev-erbβ target regions. Green and pink letters indicate the CRISPR-targeted sequence and the PAM sequence, respectively (see Methods). (b) Relative expression levels of Rev-erbα and Rev-erbβ mRNA including CRISPR-targeted exon were determined by quantitative PCR. The values were normalized to 18S rRNA and presented as means ± SD (n = 3; *p < 0.001).
