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. 2019 Jul 15;9:10230. doi: 10.1038/s41598-019-46695-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CymMV and ORSV RNA accumulation in protoplasts from WT and transgenic lines. (A) RT-qPCR analysis of CymMV and ORSV RNA in protoplasts of WT and transgenic lines after C+O infection at 18 hpi. Values are normalized against CymMV and ORSV RNA in protoplasts of WT plants infected with C+O. (B) Northern blot analysis of CymMV and ORSV in protoplasts from WT and transgenic lines transfected with C+O by DIG-labeled CP probes. Protoplasts were mock-inoculated with H₂O (Lane 1), CymMV virions (Lane 2), ORSV virions (Lane 3), and mixed C+O (Lane 4). The relative accumulation of CymMV and ORSV in transgenic lines were shown compared with single virion inoculation in WT plants.