Fig. 5.

KC-to-DAN feedback signaling is independent of ACh. a Illustration of the 1-odor reciprocal training regime used in this study (odor-sugar learning). b–e Specific knockdown of acetylcholine receptor subunits α1 (b), α4 (c), α5 (d), α6 (e), respectively, in pPAM neurons does not affect odor-sugar learning. AChR: acetylcholine receptor; dcr2: dicer2; OCT: octanol; w: w¹¹¹⁸. The number below the box plots refers to the N number. A pairwise Student’s t test or pairwise Wilcoxon test (both including Bonferroni-Holm correction) was used. Significance levels: ^n.s.p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Data are mainly presented as box plots, with 50% of the values of a given genotype being located within the box, and whiskers represent the entire set of data. No data were excluded. Outliers are indicated as open circles. The median performance index is indicated as a thick line within the box plot