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. 2019 Jul 15;9:10176. doi: 10.1038/s41598-019-46671-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The upper part: SEM image; backscattered electron image displays compositional contrast which results from different elements. The lower part: EDX analysis allows identifying these elements and their relative proportion (atomic % and weight %) by the generation of an x-ray spectrum from the entire scan area of the SEM. The Y-axis depicts the counts of x-rays and the X-axis shows their energy level (KeV). The position of the peaks identifies the elements, while the peaks height helps in the quantification of each element. Here, EDX spectrum showing significant amounts of Au (one prominent peak at 2.2 KeV with other weak peaks).