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. 2019 Jul 15;9:10236. doi: 10.1038/s41598-019-46724-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the uremic milieu (HD plasma (pl)) on healthy monocyte subpopulations compared to the effect of the healthy milieu (Ctrl plasma (pl)) on HD monocyte subpopulations. Sodium citrate blood from healthy controls (Ctrl) (n = 8) and hemodialysis patients (HD; n = 8) was centrifuged and the autologous plasma was completely removed and re-added (sham) or exchanged for blood type matched HD plasma and vice versa and incubated for 24 h. Data express monocytes subpopulations (%); upper right: zooms in on intermediate monocytes (CD14⁺⁺CD16⁺). Results are presented a mean ± SD. One significance symbol: p < 0.05, two symbols: p < 0.005 and three symbols: p < 0.001. *vs. Ctrl sham T0; ^§§vs. Ctrl sham T24; °vs. Ctrl cell + HD pl; ªvs. HD sham T0; ^#vs. HD sham T24.