Table 3.

Validation of the assembly quality and improved gene annotation of tea plant genome using three methodologies.

Validation of assembly quality	Number	Percentage (%)
BUSCO validation
Total BUSCO groups	1,440	100
Complete single-copy BUSCOs	1,180	81.9
Complete duplicated BUSCOs	151	10.5
Fragmented BUSCOs	44	3.1
Missing BUSCOs	65	4.5
BAC validation
Total BACs (#)	18	100
Total length (bp)	2,080,846	100
Aligned BACs (bp)	1,182,063	98.30
PCR validation
Total PCR experiments	24	100
Success PCR experiments	22	91.67
Validation of gene prediction
Total BUSCO groups	1,440	100
Complete single-copy BUSCOs	1,068	74.2
Complete duplicated BUSCOs	173	12.0
Fragmented BUSCOs	118	8.2
Missing BUSCOs	81	5.6

The completeness of genome assembly and gene re-annotation were evaluated using the latest version of BUSCO (v3.0.2). The result of BAC alignment and PCR validation were summarized from our previous reported tea plant genome⁶.