Table 1.
Cryo-EM Data Collection and Refinement Information.
| 112-bp octasome/Mid-pAID complex (Fig. 2A) (EMDB: 6937) | 112-bp octasome/pAID complex (Fig. 2B) (EMDB: 9639) | 112-bp hexasome (Fig. 4A) (EMDB: 6939) | 112-bp octasome/DNA complex (Fig. S14A) (EMDB: 9640) | |
|---|---|---|---|---|
| Microscope | Titan Krios | Titan Krios | Titan Krios | Talos Arctica |
| Cs corrected | Yes | Yes | Yes | No |
| Detector | Falcon II | Falcon 3EC | Falcon 3EC | Falcon 3EC |
| Volta Phase Plate | Yes | No | No | No |
| Magnification | 59,000 (nominal) | 59,000 (nominal) | 59,000 (nominal) | 73,000 (nominal) |
| Voltage (kV) | 300 | 300 | 300 | 200 |
| Electron exposure (e−/Å2) | 36 | 40 | 40 | 40 |
| Defocus range (µm) | −1.00, −1.20, −1.40 | −1.75 to −3.50 | −1.75 to −3.50 | −2.50 to −4.00 |
| Pixel size (Å) | 1.1 | 1.12 | 1.12 | 1.4 |
| Initial particle images (no.) | 2,62,319 | 4,23,626 | 2,29,216 | 5,48,421 |
| Final particle images (no.) | 30,733 | 1,32,165 | 42,515 | 37,441 |
| Map resolution (Å) | 5.7 | 4.5 | 7.5 | 7.8 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Map sharpening B factor (Å2) | −150 | −200 | −604 | −800 |