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. 2019 Jul 15;9:10216. doi: 10.1038/s41598-019-46681-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profile of phellogen-related genes in developing wound periderm. Wound periderm develops at the surface of potato tuber slices kept in the dark and under high humidity. The surface of the slices was collected at 1-d intervals after wounding. Between Day 1 and Day 3, wounding induced the formation of a closing layer and phellogen initials formed below it. On Day 3, columns of new phellem cells were added below the closing layer, and from Day 4 on, the newly formed phellem underwent suberization. The wound periderm was mature on Day 8. A detailed anatomical description is provided in Fig. S4. Gene expression during wound healing (monitored in days) was compared to that observed during two stages of native periderm development (monitored in weeks): phellogen initial stage at 3 weeks after sprout emergence (WAE) and mature skin at 12 WAE. Transcript levels were monitored by qPCR and expression levels were normalized relative to the level of the reference gene α-NAC.