Fig. 4.

Expression of Hsp27 in response to quercetin (10 and 30 μM) and resveratrol (10 and 15 μM) treatments, alone or in combination with Hsp27 si2 (25 nM) for 48 h. Western blot analysis showed that the combined treatments were much more effective in comparison to the individual compound (excepting 10 μM quercetin alone). All data were normalized to GAPDH. Graph represents the mean ± SD (n = 7). ***P < 0.001 versus untreated group. ^&&&P < 0.001 versus si2. ns indicates P > 0.05 and ^###P < 0.001, which show multiple comparisons between different groups. P values were determined by one-way ANOVA using Tukey’s multiple comparison test. si siRNA, Q quercetin, RSV resveratrol, ns not significant