FIGURE 3.

The distinct structural phenotypes of tau K18 aggregates observed in the presence of the FTD mutations do not change with extended aggregation reactions. (A) K18-WT formed PHFs and SFs, similar to those recorded in the 4 days assay in Figure 2A. (B) K18-V337M formed amorphous aggregates in addition to short filaments, similar to those observed in the 4 days aggregation assay in Figure 2B. (C) K18-N279K formed thick filament bundles whose structural features shares major similarities with those in Figure 2C. Scale bar = 100 nm for all images. Three independent experiments each performed with a different batch of purified protein.