Table 1.
Antibodies used in this study.
| Antibody | Epitope | Host species | Vendor | Dilution |
|---|---|---|---|---|
| Primary antibodies | ||||
| Polyclonal rabbit anti- human tau (#A0024) | The MT binding repeat of tau | Rabbit | Dako | 1:1000 |
| T22 oligomeric tau (#ABN454) | Tau MT binding domain | Rabbit | Merck | 1:1000 |
| HT7 anti-human tau (#MN1000) | Amino acid sequence 159PPGQK163 of full length human tau 2N4R | Mouse | Thermo Scientific | 1:100 |
| Alexa Fluor® 647-conjugated anti-nucleolin (#ab198580) | Human nucleolin | Mouse | Abcam | 1:500 |
| Secondary antibodies | ||||
| Goat anti-rabbit IgG (#31460) | – | Goat | Thermo Fisher Scientific | 1:1000 |
| Alexa Fluor® 594-conjugated AffiniPure goat anti-mouse | – | Goat | Jackson ImmunoResearch | 1:100 |