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. 2019 Jul 9;10:1499. doi: 10.3389/fmicb.2019.01499

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Copyright © 2019 Alviz-Gazitua, Fuentes-Alburquenque, Rojas, Turner, Guiliani and Seeger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complementation effects in P. aeruginosa PAO1 ΔrocR with pJBurf2.2. (A) Biofilm formation at 6 h of incubation in 96-well plates without cadmium. (B) The c-di-GMP content in planktonic cells at 6 h of incubation in Erlenmeyer flasks. The means of values of three independent experiments and standard deviations are shown. Significant differences assessed by t-test: ^∗P < 0.05, ^∗∗P < 0.01.