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. 2019 Jul 9;10:1499. doi: 10.3389/fmicb.2019.01499

Figure 5.

Figure 5

Effect of urf2.2 overexpression and pleD heterologous expression on C. metallidurans CH34 biofilm formation and c-di-GMP metabolism. (A) Biofilm formation in the absence of cadmium. The means of three independent experiments and standard deviations are shown. Significant differences assessed by t-test: P < 0.05; ns, not significant. (B) Macrocolonies morphotype in the presence of cadmium. The red staining of the macrocolony indicates its EPS content. (C) Response in c-di-GMP levels after cadmium exposure. The means of three independent experiments and standard deviations are shown. Significant differences assessed by t-test: P < 0.05, ∗∗P < 0.01; ns, non-significant.