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Sequence alignment of OjCHI with AtCHI (Arabidopsis thaliana, P41088) and MsCHI (Medicago sativa, P28012). Residues for binding (2S)-naringenin are shown with green boxes, and those for hydrogen bond network in active site are shown with blue triangles. The red star identifies residues postulated to determine substrate preference for naringenin chalcone and isoliquiritigenin.
