Skip to main content
. 2019 Jul 16;39(7):BSR20181722. doi: 10.1042/BSR20181722

Figure 3. H19 could directly regulate expression of miR-130a.

Figure 3

Relative expression level of H19 (A) and miR-130a (B) was measured by qRT-PCR in HepG2 and Huh-7 cells transfected with shH19. (C) Predicted binding site between miR-130a and H19 by TargetScan software. (D) The luciferase activity of the H19-WT and H19-MUT in HepG2 and Huh-7 cells transfected with miR-130a mimics and inhibitor. (E) PCR was used to detect H19 in the samples pulled down by biotinylated miR-130a. All the results were shown as mean ± SD (n=3), which were three separate experiments performed in triplicate. *P<0.05 and **P<0.01.