Table 3.

Differences in mucosal permeability and response to luminal glucose addition in distal jejunum of low and high residual feed intake (RFI) broiler chickens receiving either a fecal microbiota transplant (FMT), or a control transplant (CT).

	FMT		CT			P value
Item	Low RFI	High RFI	Low RFI	High RFI	SEM	FMT	RFI	FMT × RFI
Average electrophysiological parameters
FITC (nmol/cm2 × h)1	0.015	0.191	0.005	0.008	0.0883	0.283	0.320	0.335
HRP (pmol/cm2 × h)1	0.015	0.005	0.021	0.007	0.0062	0.489	0.057	0.729
Glucose response2
Basal Isc (μA/cm2)	−4.28	−3.96	−2.65	−0.69	1.872	0.200	0.548	0.665
ΔIsc	2.97	2.28	2.69	2.54	0.202	0.955	0.047	0.184
Basal RT (W/cm2)	2.29	2.03	1.92	2.18	0.435	0.795	1.000	0.553
ΔRT	0.09	0.06	0.08	0.08	0.008	0.843	0.050	0.121

Data are presented as least-square means and pooled SEM. Low RFI FMT females, n = 8; low RFI FMT males, n = 7; high RFI FMT females, n = 7; high-RFI FMT males, n = 6; low RFI CT females, n = 7; low-RFI CT males, n = 7; high-RFI CT females, n = 7; high-RFI CT males, n = 7. ¹FITC, fluorescein 5(6)-isothiocyanate; HRP, horseradish peroxidase. ²Glucose addition to a final chamber concentration of 5 mmol/l; ΔI_sc is the difference between the maximal I_sc value obtained from 2 min after glucose addition and the basal value determined 1 min before glucose addition; ΔR_T is the difference between the basal GT 1 min before glucose addition and the R_T value obtained from 2 min after glucose addition.