Table 4.

Differences in expression levels of nutrient transporter, barrier, and innate immune genes at the jejunal and cecal mucosa of low and high residual feed intake (RFI) broiler chickens receiving either a fecal microbiota transplant (FMT) or a control transplant (CT).

	FMT		CT			P value
Gene of interest	Low RFI	High RFI	Low RFI	High RFI	SEM	FMT	RFI	FMT × RFI
Jejunum
IAP	0.365	0.456	0.434	0.422	0.048	0.715	0.416	0.284
MUC1	0.127	0.120	0.056	0.088	0.051	0.318	0.809	0.700
MUC2	0.312	0.469	0.353	0.346	0.045	0.369	0.100	0.074
CLDN1	0.246	0.314	0.286	0.276	0.051	0.983	0.573	0.444
CLDN5	0.128	0.139	0.083	0.104	0.044	0.362	0.719	0.905
OCLN	0.378	0.394	0.394	0.401	0.021	0.569	0.579	0.846
ZO1	0.337	0.336	0.288	0.331	0.037	0.467	0.585	0.556
SGLT1	0.316	0.380	0.419	0.404	0.037	0.091	0.504	0.288
GLUT2	0.419	0.437	0.440	0.402	0.048	0.879	0.835	0.562
MCT1	0.129	0.127	0.120	0.125	0.017	0.750	0.942	0.853
SMCT	0.082	0.095	0.133	0.042	0.030	0.974	0.195	0.085
IL1B	0.056	0.040	0.055	0.059	0.009	0.309	0.486	0.273
IL6	0.253	0.265	0.319	0.343	0.056	0.205	0.747	0.915
IL8	0.005	0.007	0.010	0.006	0.003	0.500	0.795	0.312
IL10	0.010	0.013	0.017	0.012	0.005	0.478	0.894	0.385
TGFB1	0.169	0.123	0.103	0.118	0.037	0.347	0.687	0.411
TNFA	0.058	0.067	0.069	0.066	0.004	0.298	0.518	0.188
NFKB	0.164	0.211	0.197	0.215	0.018	0.295	0.078	0.431
TLR2	0.099	0.039	0.032	0.033	0.036	0.311	0.411	0.407
TLR4	0.308	0.270	0.314	0.343	0.038	0.307	0.909	0.385
Ceca
IAP	0.127	0.104	0.119	0.119	0.014	0.802	0.409	0.401
MUC1	0.226	0.162	0.189	0.254	0.043	0.534	0.994	0.148
MUC2	0.035	0.072	0.021	0.021	0.026	0.221	0.480	0.498
CLDN1	0.299	0.317	0.271	0.340	0.023	0.924	0.070	0.273
CLDN5	0.190	0.132	0.166	0.185	0.020	0.466	0.342	0.063
OCLN	0.468	0.531	0.536	0.479	0.056	0.889	0.948	0.290
ZO1	0.494	0.448	0.452	0.495	0.042	0.948	0.971	0.291
SGLT1	0.015	0.038	0.012	0.011	0.015	0.314	0.455	0.416
MCT1	0.556a	0.360b	0.458a,b	0.495a,b	0.055	0.743	0.154	0.039
SMCT	0.090	0.057	0.005	0.001	0.056	0.214	0.744	0.796
IL1B	0.364	0.237	0.366	0.326	0.045	0.314	0.068	0.336
IL6	0.272	0.199	0.309	0.292	0.036	0.079	0.222	0.435
IL8	0.121	0.042	0.066	0.034	0.035	0.378	0.124	0.501
IL10	0.324	0.142	0.215	0.161	0.219	0.415	0.036	0.245
TGFB1	0.324	0.316	0.310	0.293	0.033	0.580	0.710	0.887
TNFA	0.071	0.127	0.062	0.065	0.034	0.309	0.393	0.448
NFKB	0.530	0.413	0.463	0.402	0.065	0.545	0.177	0.667
TLR2	0.099	0.087	0.104	0.104	0.007	0.131	0.389	0.373
TLR4	0.569	0.716	0.553	0.549	0.140	0.515	0.610	0.591

Data are presented as least-square means and pooled SEM. Low RFI FMT females, n = 8; low RFI FMT males, n = 7; high RFI FMT females, n = 7; high-RFI FMT males, n = 6; low RFI CT females, n = 7; low-RFI CT males, n = 7; high-RFI CT females, n = 7; high-RFI CT males, n = 7. ^a,bDifferent superscripts within a row indicate significant difference (P ≤ 0.05).