Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 9;10:1558. doi: 10.3389/fmicb.2019.01558

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Panjaitan, Horng, Cheng, Chung and Soo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Overexpression of etcABC enhanced type 3 fimbriae production in K. pneumoniae. (A) The representative TEM images of bacteria incubated in LB broth in three independent experiments. KpWT/vc: K. pneumoniae STU1 carrying pBSK-Gm as the vector control. KpWT/etcABC: K. pneumoniae STU1 carrying pBSK::Gm::etcABC to overexpress etcABC. The magnifications of the images are (i) 15,000×, (ii) 50,000×, and (iii) 100,000×. (B) Transcriptional analysis of fimA, fimH, mrkA, and mrkD in KpWT/etcABC and KpWT/vc by RT-qPCR. The 16S rRNA gene was used as the reference. Relative gene expressions means amount of mrkA and mrkD mRNA in KpWT/etcABC compared to those in KpWT/vc and amount of fimA and fimH mRNA in KpWT/vc compared to those in KpWT/etcABC. The presented results are the means ± standard deviations of three replicates. The p-value of each group is less than 0.05 as compared with KpWT/vc. (C) Western blot analysis of MrkA, FimA, and ManA expression in each strain. WT: K. pneumoniae STU1, WT/vc: K. pneumoniae STU1 carrying pBSK-Gm as the vector control. WT/etcABC: K. pneumoniae STU1 carrying pBSK::Gm::etcABC to overexpress etcABC. ManA was used as the loading control. The blots are representatives of at least three independent experiments. (D) Immunogold electron microscopy using anti-MrkA was performed against K. pneumoniae STU1 carrying pBSK::Gm::etcABC. Bacteria displayed type 3 fimbriae. The magnification of the images is 50,000× (left), 100,000× (middle), and 150,000× (right), respectively.